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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRUNOL6 Polyclonal antibody (19078-1-AP)

    WB analysis using 19078-1-AP (same clone as 19078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19078-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BRUNOL6 Polyclonal antibody (19078-1-AP)

    WB analysis of HeLa using 19078-1-AP (same clone as 19078-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IHC staining of human kidney using 19078-1-AP (same clone as 19078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IHC staining of human kidney using 19078-1-AP (same clone as 19078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IF Staining of HeLa using 19078-1-AP (same clone as 19078-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19078-1-PBS in a different storage buffer formulation.

BRUNOL6 Polyclonal antibody, PBS Only

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19078-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19078-1-PBS targets BRUNOL6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4970

Product name: Recombinant human BRUNOL6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 130-481 aa of BC030835

Sequence: RGEDRKLFVGMLGKQQGEEDVRRLFQPFGHIEECTVLRSPDGTSKGCAFVKFGSQGEAQAAIRGLHGSRTMAGASSSLVVKLADTDRERALRRMQQMAGHLGAFHPAPLPLGACGAYTTAILQHQAALLAAAQGPGLGPVAAVAAQMQHVAAFSLVAAPLLPAAAANSPPGSGPGTLPGLPAPIGVNGFGPLTPQTNGQPGSDTLYNNGLSPYPAQSPGVADPLQQAYAGMHHYAAAYPSAYAPVSTAFPQQPSALPQQQREGPEGCNLFIYHLPQEFGDAELIQTFLPFGAVVSAKVFVDRATNQSKCFGFVSFDNPTSAQTAIQAMNGFQIGMKRLKAQLKRPKDANRPY

相同性解析による交差性が予測される生物種
Full Name bruno-like 6, RNA binding protein (Drosophila)
Calculated molecular weight 481 aa, 50 kDa
Observed molecular weight 50 kDa, 39 kDa
GenBank accession numberBC030835
Gene Symbol BRUNOL6
Gene ID (NCBI) 60677
RRIDAB_2243662
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96J87
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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