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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRUNOL5 Polyclonal antibody (13557-1-AP)

    WB analysis using 13557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13557-1-AP (BRUNOL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BRUNOL5 Polyclonal antibody (13557-1-AP)

    IHC staining of mouse brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BRUNOL5 Polyclonal antibody (13557-1-AP)

    IHC staining of human gliomas using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using BRUNOL5 Polyclonal antibody (13557-1-AP)

    IHC staining of rat brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using BRUNOL5 Polyclonal antibody (13557-1-AP)

    IF Staining of U-87 MG using 13557-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using BRUNOL5 antibody (13557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BRUNOL5 Polyclonal antibody

BRUNOL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13557-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U-251 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13557-1-AP targets BRUNOL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4416

Product name: Recombinant human BRUNOL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-485 aa of BC028101

Sequence: KLFVGMLNKQQSEEDVLRLFQPFGVIDECTVLRGPDGSSKGCAFVKFSSHTEAQAAIHALHGSQTMPGASSSLVVKFADTDKERTLRRMQQMVGQLGILTPSLTLPFSPYSAYAQALMQQQTTVLSTSGSYLSPGVAFSPCHIQQIGAVSLNGLPATPIAPASGLHSPPLLGTTAVPGLVAPITNGFAGVVPFPGGHPALETVYANGLVPYPAQSPTVAETLHPAFSGVQQYTAMYPTAAITPIAHSVPQPPPLLQQQQREGPEGCNLFIYHLPQEFGDTELTQMFLPFGNIISSKVFMDRATNQSKCFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDPGHPY

相同性解析による交差性が予測される生物種
Full Name bruno-like 5, RNA binding protein (Drosophila)
Calculated molecular weight 485 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC028101
Gene Symbol BRUNOL5
Gene ID (NCBI) 60680
RRIDAB_1211624
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6W0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRUNOL5, also named CELF5, is a 485 amino acid protein, which belongs to the CELF/BRUNOL family. BRUNOL5 is expressed in the brain and localizes in the nucleus. BRUNOL5 is an RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. BRUNOL5 specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. BRUNOL5 exists in two isoforms with MV 52 and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IHC protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IF protocol for BRUNOL5 antibody 13557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols