Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32257-1-AP targets BRN3A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35800 Product name: Recombinant human BRN3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 87-265 aa of NM_006237 Sequence: SVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDP 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 4 homeobox 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | NM_006237 |
| Gene Symbol | POU4F1 |
| Gene ID (NCBI) | 5457 |
| RRID | AB_3742554 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q01851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRN3A (Brain-specific Homeobox/POU domain protein 3A, also known as POU4F1) is a member of the class IV POU-domain transcription factor family. It plays a core regulatory role primarily in the development and survival of the nervous system and sensory neurons. By recognizing specific DNA sequences, it activates downstream target genes and is essential for the generation, differentiation, and maintenance of sensory ganglion neurons, including those of the trigeminal, vestibular, and auditory systems. Additionally, it is also involved in regulating cell proliferation and apoptosis in tumors such as breast cancer. It has 2 isoforms with the molecular mass of 33 kDa and 43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BRN3A antibody 32257-1-AP | Download protocol |
| IHC protocol for BRN3A antibody 32257-1-AP | Download protocol |
| WB protocol for BRN3A antibody 32257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |