Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IP | See 1 publications below |
Product Information
16096-1-AP targets BRMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8939 Product name: Recombinant human BRMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC009834 Sequence: MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP 相同性解析による交差性が予測される生物種 |
| Full Name | breast cancer metastasis suppressor 1 |
| Calculated molecular weight | 246 aa, 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC009834 |
| Gene Symbol | BRMS1 |
| Gene ID (NCBI) | 25855 |
| RRID | AB_2878217 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCU9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. BRMS1 has since been reported to suppress metastasis in a variety of cancer types such as melanoma, ovarian carcinoma, and non-small lung cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BRMS1 antibody 16096-1-AP | Download protocol |
| IHC protocol for BRMS1 antibody 16096-1-AP | Download protocol |
| WB protocol for BRMS1 antibody 16096-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Rep BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.
| ||
Oncol Res miR-346 Promotes HCC Progression by Suppressing Breast Cancer Metastasis Suppressor 1 Expression | ||
J Neurooncol Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM |