Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
84965-1-PBS targets BRD8 as part of a matched antibody pair:
MP01679-1: 84965-2-PBS capture and 84965-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BRD8 fusion protein Ag0790 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 8 |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 120-170 kDa |
| GenBank accession number | BC008039 |
| Gene Symbol | BRD8 |
| Gene ID (NCBI) | 10902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H0E9 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)