Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, mouse cerebellum tissue, mouse lung tissue, L02 cells, rat brain tissue, SH-SY5Y cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, PC-3 cells, SH-5Y5Y cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 30 publications below |
| IHC | See 12 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
| ChIP | See 2 publications below |
Product Information
51009-2-AP targets BRD7 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3911 Product name: Recombinant human BRD7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC050728 Sequence: MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGL 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 7 |
| Calculated molecular weight | 651 aa, 74 kDa |
| Observed molecular weight | 70-74 kDa |
| GenBank accession number | BC050728 |
| Gene Symbol | BRD7 |
| Gene ID (NCBI) | 29117 |
| RRID | AB_2259226 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BRD7 antibody 51009-2-AP | Download protocol |
| WB protocol for BRD7 antibody 51009-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction. | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis | ||
Cell Rep FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression | ||
Cancer Res BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription.
| ||
Cell Mol Immunol BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway.
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