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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRD4 Recombinant monoclonal antibody (88289-2-RR)

    WB analysis using 88289-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88289-2-RR (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BRD4 Recombinant monoclonal antibody (88289-2-RR)

    IF Staining of HepG2 using 88289-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (88289-2-RR, Clone: 260746D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BRD4 Recombinant monoclonal antibody

BRD4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88289-2-RR
Clone No.260746D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88289-2-RR targets BRD4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name bromodomain containing 4
Calculated molecular weight 1362 aa, 152 kDa
Observed molecular weight200 kDa
GenBank accession numberBC035266
Gene Symbol BRD4
Gene ID (NCBI) 23476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. The molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).

Protocols

Product Specific Protocols
IF protocol for BRD4 antibody 88289-2-RRDownload protocol
WB protocol for BRD4 antibody 88289-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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