Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, SH-SY5Y cells, SK-N-SH cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32452-1-AP targets BRD1/BRL in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37530 Product name: Recombinant human BRD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 918-1070 aa of BC047508 Sequence: VLPRLETLLQPRKRSRSTCGDSEVEEESPGKRLDAGLTNGFGGARSEQEPGGGLGRKATPRRRCASESSISSSNSPLCDSSFNAPKCGRGKPALVRRHTLEDRSELISCIENGNYAKAARIAAEVGQSSMWISTDAAASVLEPLKVVWAKCSG 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 1 |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | BC047508 |
| Gene Symbol | BRD1 |
| Gene ID (NCBI) | 23774 |
| RRID | AB_3742614 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O95696 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for BRD1/BRL antibody 32452-1-AP | Download protocol |
| WB protocol for BRD1/BRL antibody 32452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |