Validation Data Gallery
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Tested Applications
| Positive WB detected in | SK-BR-3 cells, HeLa cells, HEK-293T cells, MCF-7 cells, HT-29 cells, Neuro-2a cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85430-4-RR targets BRCC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BRCC3 fusion protein Ag7584 相同性解析による交差性が予測される生物種 |
| Full Name | BRCA1/BRCA2-containing complex, subunit 3 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC002999 |
| Gene Symbol | BRCC3 |
| Gene ID (NCBI) | 79184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P46736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRCC3 antibody 85430-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |