• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using BRAWNIN Polyclonal antibody (30212-1-AP)

    WB analysis using 30212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30212-1-AP (BRAWNIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using BRAWNIN Polyclonal antibody (30212-1-AP)

    IF Staining of U-251 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using BRAWNIN Polyclonal antibody (30212-1-AP)

    IF Staining of U-251 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using BRAWNIN Polyclonal antibody (30212-1-AP)

    IF Staining of A431 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

［PR］論文掲載5,600報突破！GFP免疫沈降用抗体「GFP-Trap®」を無料体験

BRAWNIN Polyclonal antibody

BRAWNIN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

BR, BRAWNIN, C12orf73, DKFZp547P055, FLJ13975, UQCC6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IF/ICC detected inU-251 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:400-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30212-1-AP targets BRAWNIN in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAWNIN fusion protein Ag32955 相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 73
Calculated molecular weight8 kDa
Observed molecular weight 9 kDa
Gene Symbol C12orf73
Gene ID (NCBI) 728568
RRIDAB_3086265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ69YU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquinol-cytochrome-c reductase complex assembly factor 6 (UQCC6) is also named as BRAWNIN, BR and C12orf73, and belongs to the UQCC6 family. BRAWNIN (BR), a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death (PMID:32161263).  BR is also detectable in human cardiac and skeletal muscle where it displays a staining pattern characteristic of the mitochondria network. BR protein abundance in mouse tissues correlated well with that of mitochondrial respiratory chain proteins, being high in brown adipose, cardiac and skeletal muscle but virtually undetectable in white adipose tissue (PMID:32161263). BR resides in the IMM and not in the outer mitochondrial membrane (OMM) (PMID:32161263).

Protocols

Product Specific Protocols
WB protocol for BRAWNIN antibody 30212-1-APDownload protocol
IF protocol for BRAWNIN antibody 30212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols