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Species Reactivity

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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human breast cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human breast cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human ovary tumor using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human ovary tumor using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human liver cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human liver cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BPTF Polyclonal antibody (28016-1-AP)

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BPTF Polyclonal antibody (28016-1-AP)

    IF Staining of HepG2 using 28016-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BPTF antibody (28016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BPTF Polyclonal antibody

BPTF Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 28016-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

FAC1, FALZ

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

28016-1-AP targets BPTF in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27761

Product name: Recombinant human BPTF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-657 aa of NM_004459

Sequence: MEPIGYDRSRRKYWFLNRRLIIEEDTENENEKKIWYYSTKVQLAELIDCLDKDYWEAELCKILEEMREEIHRHMDITEDLTNKARGSNKSFLAAANEEILESIRAKKGDIDNVKSPEETEKDKNETENDSKDAEKNREEFEDQSLEKDSDDKTPDDDPEQGKSEEPTEVGDKGNSVSANLGDNTTNATSEETSPSEGRSP

相同性解析による交差性が予測される生物種
Full Name bromodomain PHD finger transcription factor
Calculated molecular weight 338 kDa
GenBank accession numberNM_004459
Gene Symbol BPTF
Gene ID (NCBI) 2186
RRIDAB_2918139
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)

Protocols

Product Specific Protocols
IF protocol for BPTF antibody 28016-1-APDownload protocol
IHC protocol for BPTF antibody 28016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomolecules

Lumbrokinase Extracted from Earthworms Synergizes with Bevacizumab and Chemotherapeutics in Treating Non-Small Cell Lung Cancer by Targeted Inactivation of BPTF/VEGF and NF-κB/COX-2 Signaling

Authors - Chunyu Hua
View Article
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