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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BPTF Polyclonal antibody

BPTF Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28016-1-AP

Synonyms

BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301

Validation Data Gallery

Applications:
  • IHC staining of human breast cancer using 28016-1-AP

    IHC staining of human breast cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human breast cancer using 28016-1-AP

    IHC staining of human breast cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human ovary tumor using 28016-1-AP

    IHC staining of human ovary tumor using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human ovary tumor using 28016-1-AP

    IHC staining of human ovary tumor using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human colon cancer using 28016-1-AP

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human colon cancer using 28016-1-AP

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human liver cancer using 28016-1-AP

    IHC staining of human liver cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human liver cancer using 28016-1-AP

    IHC staining of human liver cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human colon cancer using 28016-1-AP

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IHC staining of human colon cancer using 28016-1-AP

    IHC staining of human colon cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IF Staining of HepG2 using 28016-1-AP

    IF Staining of HepG2 using 28016-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BPTF antibody (28016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BPTF Ready-To-Use IHC Kit. BPTF primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28016-1-AP targets BPTF in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPTF fusion protein Ag27761 相同性解析による交差性が予測される生物種
Full Name bromodomain PHD finger transcription factor
Calculated molecular weight 338 kDa
GenBank accession numberNM_004459
Gene symbol BPTF
Gene ID (NCBI) 2186
RRIDAB_2918139
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)

Protocols

Product Specific Protocols
IHC protocol for BPTF antibody 28016-1-APDownload protocol
IF protocol for BPTF antibody 28016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols