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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HEK-293 using 16461-1-AP

    HEK-293 and Hela cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HeLa using 16461-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of human kidney using 16461-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HEK-293 using 16461-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BPNT1 Polyclonal antibody (16461-1-AP)

    IF Staining of HeLa using 16461-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16461-1-AP (BPNT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BPNT1 Polyclonal antibody

BPNT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16461-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Bisphosphate 3 nucleotidase 1, BPNT1, PAP inositol 1,4 phosphatase, PIP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16461-1-AP targets BPNT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9523

Product name: Recombinant human BPNT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC017801

Sequence: MASSNTVLMRLVASAYSIAQKAGMIVRRVIAEGDLGIVEKTCATDLQTKADRLAQMSICSSLARKFPKLTIIGEEDLPSEEVDQELIEDSQWEEILKQPCPSQYSAIKEEDLVVWVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGVINQPYYNYEAGPDAVLGRTIWGVLGLGAFGFQLKEVPAGKHIITTTRSHSNKLVTDCVAAMNPDAVLRVGGVGNKIIQLIEGKASAYVFASPGCKKWDTCAPEVILHAVGGKLTDIHGNVLQYHKDVSHRTWPKPDFFRAQFFLESHSCFSRNFKNVSTPIKNIYDVIIYAYETDL

相同性解析による交差性が予測される生物種
Full Name 3'(2'), 5'-bisphosphate nucleotidase 1
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC017801
Gene Symbol BPNT1
Gene ID (NCBI) 10380
RRIDAB_2067193
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95861
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for BPNT1 antibody 16461-1-APDownload protocol
IHC protocol for BPNT1 antibody 16461-1-APDownload protocol
WB protocol for BPNT1 antibody 16461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Mammalian tRNA acetylation determines translation efficiency and tRNA quality control.

Authors - Na Liu
View Article
ratWB

PLoS One

Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea

Authors - Jing Liu
View Article
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