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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BPHL Polyclonal antibody

BPHL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17974-1-AP

Synonyms

BPH RP, BPHL, MCNAA, VACVASE, Valacyclovir hydrolase, Valacyclovirase

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 17974-1-AP

    WB analysis of human kidney using 17974-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 17974-1-AP

    WB analysis of human liver using 17974-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 17974-1-AP

    WB analysis of mouse kidney using 17974-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 17974-1-AP

    WB analysis of mouse kidney using 17974-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 17974-1-AP

    WB analysis of MCF-7 using 17974-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17974-1-AP

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 17974-1-AP

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human placenta using 17974-1-AP

    IHC staining of human placenta using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 17974-1-AP

    IHC staining of human placenta using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 17974-1-AP

    IHC staining of human lung using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 17974-1-AP

    IHC staining of human lung using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary using 17974-1-AP

    IHC staining of human ovary using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 17974-1-AP

    IHC staining of human ovary using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human heart using 17974-1-AP

    IHC staining of human heart using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 17974-1-AP

    IHC staining of human heart using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman kidney tissue, human liver tissue, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17974-1-AP targets BPHL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPHL fusion protein Ag12085 相同性解析による交差性が予測される生物種
Full Name biphenyl hydrolase-like (serine hydrolase)
Calculated molecular weight 22 kDa, 32 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC037778
Gene symbol BPHL
Gene ID (NCBI) 670
RRIDAB_2274994
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BPHL antibody 17974-1-APDownload protocol
IHC protocol for BPHL antibody 17974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols