Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse liver using 17173-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 17173-1-AP
human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 17173-1-AP
human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17173-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 17173-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 17173-1-AP
IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.
IHC staining of human liver cancer using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

BPGM Polyclonal antibody

BPGM Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17173-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

17173-1-AP targets BPGM in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10731 Product name: Recombinant human BPGM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC017050 Sequence: MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK 相同性解析による交差性が予測される生物種
Full Name	2,3-bisphosphoglycerate mutase
Calculated molecular weight	259 aa, 30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC017050
Gene Symbol	BPGM
Gene ID (NCBI)	669
RRID	AB_2274991
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07738
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BPGM antibody 17173-1-AP	Download protocol
IHC protocol for BPGM antibody 17173-1-AP	Download protocol
IP protocol for BPGM antibody 17173-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IP	Cell Metab Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis. Authors - Ping Xu View Article
rat	WB,IF	Brain Res Bull Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Authors - Guoji E View Article
rat	WB	Front Physiol Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia Authors - Song Yu View Article
human	WB	Aging Cell Longevity Humans Have Youthful Erythrocyte Function and Metabolic Signatures Authors - Fang Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 17173-1-AP

WB analysis of human plasma using 17173-1-AP

WB analysis of human plasma using 17173-1-AP

WB analysis of mouse liver using 17173-1-AP

WB analysis of rat liver using 17173-1-AP

IP experiment of mouse liver using 17173-1-AP

IHC staining of human liver cancer using 17173-1-AP

IHC staining of human liver cancer using 17173-1-AP

IHC staining of human liver using 17173-1-AP