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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BOULE Polyclonal antibody (13720-1-AP)

    WB analysis using 13720-1-AP (same clone as 13720-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using BOULE Polyclonal antibody (13720-1-AP)

    IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using BOULE Polyclonal antibody (13720-1-AP)

    IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using BOULE Polyclonal antibody (13720-1-AP)

    IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 13720-1-AP (BOULE antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

BOULE Polyclonal antibody, PBS Only

BOULE Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 13720-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

BOLL, Protein boule-like

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13720-1-PBS targets BOULE in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4662

Product name: Recombinant human BOLL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC033674

Sequence: MQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY

相同性解析による交差性が予測される生物種
Full Name bol, boule-like (Drosophila)
Calculated molecular weight 283 aa, 31 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC033674
Gene Symbol BOULE
Gene ID (NCBI) 66037
RRIDAB_2290617
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9W6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

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