Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
27904-1-AP targets BORG5/CDC42EP1 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BORG5/CDC42EP1 fusion protein Ag27396 相同性解析による交差性が予測される生物種 |
| Full Name | CDC42 effector protein (Rho GTPase binding) 1 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC009356 |
| Gene Symbol | CDC42EP1 |
| Gene ID (NCBI) | 11135 |
| RRID | AB_3086008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00587 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC42EP1, also known as BORG5, is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. This protein is secreted and is primarily found in bone marrow. BORG5 was discovered as a 55 kDa protein, but the accepted reference protein is only 40.3 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BORG5/CDC42EP1 antibody 27904-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci Borg5/Cdc42EP1 restricts contractility and motility in epithelial MDCK cells
|