Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, RT-4 cells, U-251 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33196-1-AP targets BORC6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37224 Product name: Recombinant human C17orf59 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-223 aa of BC018880 Sequence: MDVEQQEEEDNDEEAAAGSRAGRSFSSRLQDSRSLDGLSEACGGAGSSGSAESGAGGGRRATISSPLELEGTVSRHGDLTHFVANNLQLKIRLSGAPPPPPSAPARPCPAPAPTPTPAIPPIDPEVLRDLERLSRELGGRVDRLLRGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRRTLEVLEALCK 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 17 open reading frame 59 |
| Calculated molecular weight | 357 aa, 37 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC018880 |
| Gene Symbol | C17orf59 |
| Gene ID (NCBI) | 54785 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96GS4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BORC6 is a part of the BORC complex, which may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PMID: 25898167).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BORC6 antibody 33196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |