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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BOP1 Recombinant antibody (82742-3-RR)

    WB analysis using 82742-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BOP1 Recombinant antibody (82742-3-RR)

    WB analysis of HeLa using 82742-3-RR

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using BOP1 Recombinant antibody (82742-3-RR)

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using BOP1 Recombinant antibody (82742-3-RR)

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BOP1 Recombinant antibody (82742-3-RR)

    IF Staining of HepG2 using 82742-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BOP1 Recombinant antibody (82742-3-RR)

    IF Staining of HeLa using 82742-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Featured Product
  • KD/KO Validated

BOP1 Recombinant monoclonal antibody

BOP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82742-3-RR
Clone No.3A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0124, block of proliferation 1, 3A4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, HeLa cells, DU 145 cells, PC-3 cells, U2OS celss
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82742-3-RR targets BOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26669

Product name: Recombinant human BOP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC013787

Sequence: MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNT

相同性解析による交差性が予測される生物種
Full Name block of proliferation 1
Calculated molecular weight 84 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC013787
Gene Symbol BOP1
Gene ID (NCBI) 23246
RRIDAB_3086524
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).

Protocols

Product Specific Protocols
WB protocol for BOP1 antibody 82742-3-RRDownload protocol
IHC protocol for BOP1 antibody 82742-3-RRDownload protocol
IF protocol for BOP1 antibody 82742-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols