Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, mouse brain cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29848-1-AP targets BNIP3L in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31514 Product name: Recombinant human BNIP3L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-146 aa of BC001559 Sequence: DAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSS 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3-like |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC001559 |
| Gene Symbol | BNIP3L |
| Gene ID (NCBI) | 665 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BNIP3L (also known as NIX) is a mitochondrial outer-membrane protein that belongs to the BH3-only subgroup of the BCL-2 family. Initially identified as a weak pro-apoptotic factor, it has emerged as a key mitophagy receptor that bridges damaged or surplus mitochondria to the autophagosome, thereby safeguarding mitochondrial quality and cellular homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BNIP3L antibody 29848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |