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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using BNIP3 Monoclonal antibody (68091-1-Ig)

    WB analysis of NIH/3T3 using 68091-1-Ig (same clone as 68091-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BNIP3 Monoclonal antibody (68091-1-Ig)

    WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using BNIP3 Monoclonal antibody (68091-1-Ig)

    WB analysis of HSC-T6 using 68091-1-Ig (same clone as 68091-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BNIP3 Monoclonal antibody (68091-1-Ig)

    IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BNIP3 Monoclonal antibody (68091-1-Ig)

    IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using BNIP3 Monoclonal antibody (68091-1-Ig)

    IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using BNIP3 Monoclonal antibody (68091-1-Ig)

    IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BNIP3 Monoclonal antibody (68091-1-Ig)

    IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BNIP3 Monoclonal antibody (68091-1-Ig)

    IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

BNIP3 Monoclonal antibody, PBS Only

BNIP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68091-1-PBS
Clone No.2H7D8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2H7D8, NIP3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68091-1-PBS targets BNIP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27935

Product name: Recombinant human BNIP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-194 aa of BC009342

Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF

相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 3
Calculated molecular weight 194 aa, 22 kDa
Observed molecular weight25-30,50-60 kDa
GenBank accession numberBC009342
Gene Symbol BNIP3
Gene ID (NCBI) 664
RRIDAB_2918828
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ12983
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

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