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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BNIP2 Polyclonal antibody (10361-1-AP)

    WB analysis using 10361-1-AP (same clone as 10361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10361-1-AP (BNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10361-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BNIP2 Polyclonal antibody (10361-1-AP)

    IF Staining of HeLa using 10361-1-AP (same clone as 10361-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10361-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using BNIP2 Polyclonal antibody (10361-1-AP)

    IF Staining of RAW 264.7 using 10361-1-AP (same clone as 10361-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10361-1-PBS in a different storage buffer formulation.

BNIP2 Polyclonal antibody, PBS Only

BNIP2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 10361-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NIP2, BNIP 2, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10361-1-PBS targets BNIP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0403

Product name: Recombinant human BNIP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-313 aa of BC002461

Sequence: MKAIEPYKKVISHGGYYGDGLNAIVVFAVCFMPESSQPNYRYLMDNLFKYVIGTLELLVAENYMIVYLNGATTRRKMPSLGWLRKCYQQIDRRLRKNLKSLIIVHPSWFIRTLLAVTRPFISSKFSQKIRYVFNLAELAELVPMEYVGIPECIKQVDQELNGKQDEPKNE

相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002461
Gene Symbol BNIP2
Gene ID (NCBI) 663
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12982
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) is also named as PRKACN2. BNIP2 is developmentally regulated may suggest a role of this gene in those brain areas where the differentiation is orchestrated by estradiol. The expression of BNIP2 was inhibited by estradiol. BNIP2 is implicated in the suppression of cell death and interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. BNIP2 is highly expressed in patients with favorable neuroblastoma (NB) (PMID: 25611382).

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