Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse uterus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IP | See 2 publications below |
Product Information
55220-1-AP targets BNC2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | basonuclin 2 |
| Calculated molecular weight | 122 kDa |
| Observed molecular weight | 122-140 kDa |
| GenBank accession number | NM_017637 |
| Gene Symbol | BNC2 |
| Gene ID (NCBI) | 54796 |
| RRID | AB_2881290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BNC2 antibody 55220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis
| ||
Cell Death Dis O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in myofibroblasts |