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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    WB analysis of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    WB analysis of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IP experiment of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    IP result of anti-BMPR2 (IP:19087-1-AP, 4ug; Detection:19087-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using BMPR2 Polyclonal antibody (19087-1-AP)

    FC experiment of PC-3 using 19087-1-AP (same clone as 19087-1-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug BMPR2 Polyclonal antibody (19087-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BMPR2 Polyclonal antibody, PBS Only

BMPR2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 19087-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BMPR-2, BMPR II, BMPR 2, BMP type-2 receptor, BMP type II receptor

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19087-1-PBS targets BMPR2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5868

Product name: Recombinant human BMPR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 172-504 aa of BC052985

Sequence: YRMLTGDRKQGLHSMNMMEAAASEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVSSCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTGNRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEIFMRCTDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEARLTAQCAEERMAEL

相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type II (serine/threonine kinase)
Calculated molecular weight 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC052985
Gene Symbol BMPR2
Gene ID (NCBI) 659
RRIDAB_10603355
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13873
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. The dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form, and 150 kD dimer.

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