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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMPR1B Polyclonal antibody (26696-1-AP)

    WB analysis using 26696-1-AP (same clone as 26696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26696-1-AP (BMPR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BMPR1B Polyclonal antibody (26696-1-AP)

    IF Staining of MCF-7 using 26696-1-AP (same clone as 26696-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BMPR1B antibody (26696-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26696-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26696-1-PBS targets BMPR1B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMPR1B fusion protein Ag24902 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type IB
Calculated molecular weight 57 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC047773
Gene Symbol BMPR1B
Gene ID (NCBI) 658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00238
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BMPR1B (Bone morphogenetic protein receptor type-1B) is also named as CDw293. BMPR1B belongs to the protein kinase superfamily. BMPR1B is on ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. BMPR1B is receptor for BMP7/OP-1 and GDF5. BMPR1B Positively regulates chondrocyte differentiation through GDF5 interaction.