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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of mouse lung using 13253-1-AP (same clone as 13253-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of mouse lung using 13253-1-AP (same clone as 13253-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of HUVEC using 13253-1-AP (same clone as 13253-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP (same clone as 13253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP (same clone as 13253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP (same clone as 13253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP (same clone as 13253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13253-1-PBS in a different storage buffer formulation.

BMP5 Polyclonal antibody, PBS Only

BMP5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13253-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

BMP 5, BMP-5, Bone morphogenetic protein 5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13253-1-PBS targets BMP5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3952

Product name: Recombinant human BMP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-348 aa of BC027958

Sequence: GLGDNHVHSSFIYRRLRNHERREIQREILSILGLPHRPRPFSPGKQASSAPLFMLDLYNAMTNEENPEESEYSVRASLAEETRGARKGYPASPNGYPRRIQLSRTTPLTTQSPPLASLHDTNFLNDADMVMSFVNLVERDKDFSHQRRHYKEFRFDLTQIPHGEAVTAAEFRIYKDRSNNRFENETIKISIYQIIKEYTNRDADLFLLDTRKAQALDVGWLVFDITVTSNHWVINPQNNLGLQLCAETGDGRSINVKSAGLVGRQGPQSKQPFMVAFFKASEVLLRSVRAANKRKNQNRNKSSSHQDSSRMSSVGDYNTSE

相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein 5
Calculated molecular weight 454 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC027958
Gene Symbol BMP5
Gene ID (NCBI) 653
RRIDAB_2877929
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP22003
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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