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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using BMP3 Polyclonal antibody (22188-1-AP)

    WB analysis of Recombinant protein using 22188-1-AP (same clone as 22188-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22188-1-AP (BMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human liver cancer using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human liver cancer using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human lung cancer using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human lung cancer using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human liver using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using BMP3 Polyclonal antibody (22188-1-AP)

    IHC staining of human liver using 22188-1-AP (same clone as 22188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22188-1-PBS in a different storage buffer formulation.

BMP3 Polyclonal antibody, PBS Only

BMP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22188-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

BMP 3, BMP 3A, BMP-3, BMP3A, BMP-3A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22188-1-PBS targets BMP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16750

Product name: Recombinant human BMP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 22-349 aa of BC096269

Sequence: GERPKPPFPELRKAVPGDRTAGGGPDSELQPQDKVSEHMLRLYDRYSTVQAARTPGSLEGGSQPWRPRLLREGNTVRSFRAAAAETLERKGLYIFNLTSLTKSENILSATLYFCIGELGNISLSCPVSGGCSHHAQRKHIQIDLSAWTLKFSRNQSQLLGHLSVDMAKSHRDIMSWLSKDITQFLRKAKENEEFLIGFNITSKGRQLPKRRLPFPEPYILVYANDAAISEPESVVSSLQGHRNFPTGTVPKWDSHIRAALSIERRKKRSTGVLLPLQNNELPGAEYQYKKDEVWEERKPYKTLQAQAPEKSKNKKKQRKGPHRKSQTL

相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein 3
Calculated molecular weight 472 aa, 53 kDa
GenBank accession numberBC096269
Gene Symbol BMP3
Gene ID (NCBI) 651
RRIDAB_2879019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12645
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.

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