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Validation Data Gallery

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  • Western Blot (WB) analysis of pig bone marrow tissue using BMP2 Monoclonal antibody (66383-1-Ig)

    WB analysis of pig bone marrow using 66383-1-Ig (same clone as 66383-1-PBS)

    pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BMP2 Monoclonal antibody (66383-1-Ig)

    WB analysis using 66383-1-Ig (same clone as 66383-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BMP2 Monoclonal antibody (66383-1-Ig)

    WB analysis of HeLa using 66383-1-Ig (same clone as 66383-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using BMP2 Monoclonal antibody (66383-1-Ig)

    WB analysis of Caco-2 using 66383-1-Ig (same clone as 66383-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using BMP2 Monoclonal antibody (66383-1-Ig)

    IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using BMP2 Monoclonal antibody (66383-1-Ig)

    IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

BMP2 Monoclonal antibody, PBS Only

BMP2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66383-1-PBS
Clone No.1C5C2

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66383-1-PBS targets BMP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13501

Product name: Recombinant human BMP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC069214

Sequence: MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTSLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein 2
Calculated molecular weight 396 aa, 44 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC069214
Gene Symbol BMP2
Gene ID (NCBI) 650
RRIDAB_2881759
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12643
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).

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