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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMI1 Recombinant monoclonal antibody (87836-1-RR)

    WB analysis using 87836-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87836-1-RR (BMI1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BMI1 Recombinant monoclonal antibody (87836-1-RR)

    WB analysis of HEK-293 using 87836-1-RR

    WB result of BMI1 antibody (87836-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMI1 transfected HEK-293 cells.

  • Affinity of 87836-1-RR

    Affinity of 87836-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87836-1-RR against Human BMI1 were performed. The affinity constant is below 1 pM.

  • KD/KO Validated

BMI1 Recombinant monoclonal antibody

BMI1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87836-1-RR
Clone No.253339C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, A549 cells, SW480 cells, Calu-1 cells, NIH/3T3 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87836-1-RR targets BMI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1286

Product name: Recombinant human BMI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-326 aa of BC011652

Sequence: MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

相同性解析による交差性が予測される生物種
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight41-43 kDa
GenBank accession numberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
RRIDAB_3745777
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Protocols

Product Specific Protocols
WB protocol for BMI1 antibody 87836-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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