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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis using 10775-1-AP (same clone as 10775-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis of mouse liver using 10775-1-AP (same clone as 10775-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis of L02 using 10775-1-AP (same clone as 10775-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP (same clone as 10775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human pancreas using 10775-1-AP (same clone as 10775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human pancreas using 10775-1-AP (same clone as 10775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP (same clone as 10775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP (same clone as 10775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using BLVRA Polyclonal antibody (10775-1-AP)

    IF Staining of L02 using 10775-1-AP (same clone as 10775-1-PBS)

    Immunofluorescent analysis of L02 cells using 10775-1-AP (BLVRA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10775-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BLVRA Polyclonal antibody, PBS Only

BLVRA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10775-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10775-1-PBS targets BLVRA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1166

Product name: Recombinant human BLVRA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-296 aa of BC008456

Sequence: MNAEPERKFGVVVVGVGRAGSVRMRDLRNPHPSSAFLNLIGFVSRRELGSIDGVQQISLEDALSSQEVEVAYICSESSSHEDYIRQFLNAGKHVLVEYPMTLSLAAAQELWELAEQKGKVLHEEHVELLMEEFAFLKKEVVGKDLLKGSLLFTAGPLEEERFGFPAFSGISRLTWLVSLFGELSLVSATLEERKEDQYMKMTVCLETEKKSPLSWIEEKGPGLKRNRYLSFHFKSGSLENVPNVGVNKNIFLKDQNIFVQKLLGQFSEKELAAEKKRILHCLGLAEEIQKYCCSRK

相同性解析による交差性が予測される生物種
Full Name biliverdin reductase A
Calculated molecular weight33.2 kDa
Observed molecular weight 37-40 kDa, 66 kDa
GenBank accession numberBC008456
Gene Symbol BLVRA
Gene ID (NCBI) 644
RRIDAB_2065073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP53004
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BLVRA(Biliverdin reductase A) is also named as BLVR, BVR and belongs to the biliverdin reductase subfamily.It catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.This protein can exsit as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

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