Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14245-1-AP (same clone as 14245-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
WB analysis of U-937 using 14245-1-AP (same clone as 14245-1-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)
IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

BIN2 Polyclonal antibody, PBS Only

BIN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14245-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14245-1-PBS targets BIN2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5502 Product name: Recombinant human BIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-565 aa of BC047686 Sequence: LRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISADSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL 相同性解析による交差性が予測される生物種
Full Name	bridging integrator 2
Calculated molecular weight	62 kDa
Observed molecular weight	80-90 kDa
GenBank accession number	BC047686
Gene Symbol	BIN2
Gene ID (NCBI)	51411
RRID	AB_2063647
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBW5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14245-1-AP (same clone as 14245-1-PBS)

WB analysis of U-937 using 14245-1-AP (same clone as 14245-1-PBS)

WB analysis of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)