Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 14647-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14647-1-AP
WB result of BIN1 antibody (14647-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BIN1 transfected HeLa cells.
WB analysis of mouse brain using 14647-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14647-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14647-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human osteosarcoma using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human osteosarcoma using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse brain using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14647-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 14647-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 14647-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 14647-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

AMPH2, Amphiphysin II, Amphiphysin like protein, Amphiphysin-like protein, AMPHL

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected in	mouse skeletal muscle tissue, human osteosarcoma tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 7 publications below
CoIP	See 1 publications below

Product Information

14647-1-AP targets BIN1 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6240 Product name: Recombinant human BIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-439 aa of BC004101 Sequence: MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLRAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP 相同性解析による交差性が予測される生物種
Full Name	bridging integrator 1
Calculated molecular weight	65 kDa
Observed molecular weight	50-65 kDa
GenBank accession number	BC004101
Gene Symbol	BIN1
Gene ID (NCBI)	274
RRID	AB_2243396
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00499
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

Protocols

Product Specific Protocols
IF protocol for BIN1 antibody 14647-1-AP	Download protocol
IHC protocol for BIN1 antibody 14647-1-AP	Download protocol
WB protocol for BIN1 antibody 14647-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia Authors - Alena Kozlova View Article
mouse,human	WB	Mol Neurodegener BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia. Authors - Ari Sudwarts View Article
mouse	WB	Autophagy BIN1 deficiency enhances ULK3-dependent autophagic flux and reduces dendritic size in mouse hippocampal neurons Authors - Yuxi Jin KD Validated View Article
		Adv Sci (Weinh) BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation. Authors - Jianbo Liu View Article
human	WB,IHC	Cell Death Discov Long non-coding RNA SNHG10 upregulates BIN1 to suppress the tumorigenesis and epithelial-mesenchymal transition of epithelial ovarian cancer via sponging miR-200a-3p. Authors - Wei Lv View Article
human	WB,CoIP	Oncogene SRSF10 promotes cisplatin resistance in bladder cancer via BIN1 Exon 12 retention and ANXA1 activation Authors - Mengqing Xiao View Article

Validation Data Gallery

WB analysis using 14647-1-AP

WB analysis of HeLa using 14647-1-AP

WB analysis of mouse brain using 14647-1-AP

WB analysis of Jurkat using 14647-1-AP

WB analysis of mouse skeletal muscle using 14647-1-AP

IHC staining of mouse skeletal muscle using 14647-1-AP

IHC staining of mouse skeletal muscle using 14647-1-AP

IHC staining of human osteosarcoma using 14647-1-AP

IHC staining of human osteosarcoma using 14647-1-AP

IHC staining of mouse brain using 14647-1-AP

IHC staining of mouse brain using 14647-1-AP

IF Staining of mouse brain using 14647-1-AP

IF Staining of mouse brain using 14647-1-AP

BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia

BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.

BIN1 deficiency enhances ULK3-dependent autophagic flux and reduces dendritic size in mouse hippocampal neurons

BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation.

Long non-coding RNA SNHG10 upregulates BIN1 to suppress the tumorigenesis and epithelial-mesenchymal transition of epithelial ovarian cancer via sponging miR-200a-3p.

SRSF10 promotes cisplatin resistance in bladder cancer via BIN1 Exon 12 retention and ANXA1 activation

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