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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    WB analysis of mouse liver using 16351-1-AP (same clone as 16351-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16351-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    WB analysis of rat liver using 16351-1-AP (same clone as 16351-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    IHC staining of human kidney using 16351-1-AP (same clone as 16351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    IHC staining of human kidney using 16351-1-AP (same clone as 16351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16351-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16351-1-PBS targets BHMT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BHMT2 fusion protein Ag9558 相同性解析による交差性が予測される生物種
Full Name betaine-homocysteine methyltransferase 2
Calculated molecular weight 363 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC020665
Gene Symbol BHMT2
Gene ID (NCBI) 23743
RRIDAB_2878248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2M3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.