Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse kidney using 15965-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 15965-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15965-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 15965-1-AP
IP result of anti-BHMT (IP:15965-1-AP, 4ug; Detection:15965-1-AP 1:1000) with mouse kidney tissue lysate 4400ug.
IHC staining of human spleen using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 15965-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

BHMT Polyclonal antibody

BHMT Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15965-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IP, ELISA

Synonyms

BHMT

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, human kidney tissue, L02 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human spleen tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 35 publications below
IHC	See 2 publications below

Product Information

15965-1-AP targets BHMT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BHMT fusion protein Ag8738 相同性解析による交差性が予測される生物種
Full Name	betaine-homocysteine methyltransferase
Calculated molecular weight	406 aa, 45 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC012616
Gene Symbol	BHMT
Gene ID (NCBI)	635
RRID	AB_2290472
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q93088
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BHMT antibody 15965-1-AP	Download protocol
IHC protocol for BHMT antibody 15965-1-AP	Download protocol
IP protocol for BHMT antibody 15965-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
mouse	WB	NPJ Sci Food Ablation of the gut microbiota alleviates high-methionine diet-induced hyperhomocysteinemia and glucose intolerance in mice Authors - Wenqiang Li View Article
	WB	J Anim Sci Biotechnol Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression. Authors - Nagmeldin A Omer View Article
rat	WB	J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. Authors - Shu Yang View Article
pig	WB	J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Authors - Yimin Jia View Article
pig	WB	Sci Rep Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries. Authors - Longfei Jia View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 15965-1-AP

WB analysis of L02 using 15965-1-AP

WB analysis of human kidney using 15965-1-AP

IP experiment of mouse kidney using 15965-1-AP

IHC staining of human spleen using 15965-1-AP

IHC staining of human spleen using 15965-1-AP

IHC staining of human lung using 15965-1-AP

IHC staining of human heart using 15965-1-AP

IHC staining of human heart using 15965-1-AP

IHC staining of human placenta using 15965-1-AP

IHC staining of human placenta using 15965-1-AP

IHC staining of human skin using 15965-1-AP

IHC staining of human skin using 15965-1-AP

IHC staining of human lung using 15965-1-AP

IHC staining of human ovary using 15965-1-AP

IHC staining of human ovary using 15965-1-AP

BHMT Polyclonal antibody

BHMT Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Ablation of the gut microbiota alleviates high-methionine diet-induced hyperhomocysteinemia and glucose intolerance in mice

Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries.