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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Filensin Polyclonal antibody (17492-1-AP)

    WB analysis using 17492-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17492-1-AP (Filensin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Filensin Polyclonal antibody (17492-1-AP)

    IF Staining of HeLa using 17492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Filensin antibody (17492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Filensin Polyclonal antibody

Filensin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17492-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

BFSP1, LIFL-H, Lens intermediate filament-like heavy, Lens fiber cell beaded-filament structural protein CP 115, Filensin N-terminal fragment

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17492-1-AP targets Filensin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Filensin fusion protein Ag11591 相同性解析による交差性が予測される生物種
Full Name beaded filament structural protein 1, filensin
Calculated molecular weight540aa,60 kDa; 665aa,75 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC041483
Gene Symbol Filensin
Gene ID (NCBI) 631
RRIDAB_2228016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12934
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Filensin antibody 17492-1-APDownload protocol
IF protocol for Filensin antibody 17492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols