Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, HEK-293 cells, HepG2 cells, U2OS cells, mouse embryo |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87001-4-RR targets BEND3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag19137 Product name: Recombinant human BEND3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 493-828 aa of BC131628 Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK 相同性解析による交差性が予測される生物種 |
| Full Name | BEN domain containing 3 |
| Calculated molecular weight | 828 aa, 94 kDa |
| Observed molecular weight | 94-110 kDa |
| GenBank accession number | BC131628 |
| Gene Symbol | BEND3 |
| Gene ID (NCBI) | 57673 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5T5X7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BEND3 antibody 87001-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |