Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24958-1-AP targets BEND2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21152 Product name: Recombinant human BEND2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 298-645 aa of BC096745 Sequence: FHPNLESGPQMSYGTMSYSTEMKNNCDQDDASASACLTPDFALLPLNILVEVDTNTENSVNTMNRSTLLDSDSGQDSSSSSVCIPPKYGYLGDPKRNVRVLKIHLLAVQNMAKPKQAACYLVRILFSKEILISSSVDIHLKDSQSLDPNKMAALREYLATTFPTCDLHEHGKDWQDCISGINSMIYCLCSEGKSTPKTVRKNKKRTNRVASASADRNDQRGRDGGEGCSWMFQPMNNSKMREKRNLQPNSNAIPEGMREPSTDNPEEPGEAWSYFGRPWRNIRMPCSVLTLAKTKSCASLSARYLIQKLFTKDVLVQSNVYGNLKHGLCALDPNKISALRGLLHIEYV 相同性解析による交差性が予測される生物種 |
| Full Name | BEN domain containing 2 |
| Calculated molecular weight | 799 aa, 88 kDa |
| Observed molecular weight | 130-140 kDa |
| GenBank accession number | BC096745 |
| Gene Symbol | BEND2 |
| Gene ID (NCBI) | 139105 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BEND2 (BEN domain-containing protein 2) is a novel protein that is specifically expressed in spermatogenic cells around the time of meiotic initiation (PMID: 35613276). BEND2 interacted with a number of chromatin-associated proteins-including ZMYM2, LSD1, CHD4, and ADNP- which are components of certain transcription-repressor complexes. In the testis, Bend2 expresses two protein isoforms: the 130 kDa protein corresponds to the full-length BEND2, which displays a slower electrophoretic mobility due to its unusual sequence/structure. The 75 kDa protein corresponds to a smaller version of BEND2 lacking the N-terminus, p80 .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BEND2 antibody 24958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |