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Species Reactivity

Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEGAIN Polyclonal antibody (14956-1-AP)

    WB analysis using 14956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14956-1-AP (BEGAIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BEGAIN Polyclonal antibody

BEGAIN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14956-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BEGAIN, KIAA1446

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, JAR cells, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14956-1-AP targets BEGAIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6794

Product name: Recombinant human BEGAIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-593 aa of BC002607

Sequence: DIYCSDTALYCPEERRRDRRPSVDAPVTDVGFLRAQNSTDSAAEEEEEAEAAAFPAGFQHEAFPSYAGSLPTSSSYSSFSATSEEKEHAQASTLTASQQAIYLNSRDELFDRKPPATTYEGSPRFAKATAAVAAPLEAEVAPGFGRTMSPYPAETFRFPASPGPQQALMPPNLWSLRAKPGTARLPGEDMRGQWRPLSVEDIGAYSYPVSAAGRASPCSFSERYYGGAGGSPGKKADGRASPLYASYKADSFSEGDDLSQGHLAEPCFLRAGGDLSLSPGRSADPLPGYAPSEGGDGDRLGVQLCGTASSPEPEQGSRDSLEPSSMEASPEMHPAARLSPQQAFPRTGGSGLSRKDSLTKAQLYGTLLN

相同性解析による交差性が予測される生物種
Full Name brain-enriched guanylate kinase-associated homolog (rat)
Calculated molecular weight 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC002607
Gene Symbol BEGAIN
Gene ID (NCBI) 57596
RRIDAB_2064487
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for BEGAIN antibody 14956-1-APDownload protocol
WB protocol for BEGAIN antibody 14956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

The First Nationwide Survey and Genetic Analyses of Bardet-Biedl Syndrome in Japan.

Authors - Makito Hirano
View Article
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