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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEGAIN Polyclonal antibody (14956-1-AP)

    WB analysis using 14956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14956-1-AP (BEGAIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse pancreas using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human stomach cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse adrenal gland using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of rat adrenal gland using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BEGAIN Polyclonal antibody

BEGAIN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14956-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Brain-enriched guanylate kinase-associated protein, KIAA1446

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, JAR cells, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, mouse adrenal gland tissue, rat adrenal gland tissue, mouse brain tissue, mouse cerebellum tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14956-1-AP targets BEGAIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6794

Product name: Recombinant human BEGAIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-593 aa of BC002607

Sequence: DIYCSDTALYCPEERRRDRRPSVDAPVTDVGFLRAQNSTDSAAEEEEEAEAAAFPAGFQHEAFPSYAGSLPTSSSYSSFSATSEEKEHAQASTLTASQQAIYLNSRDELFDRKPPATTYEGSPRFAKATAAVAAPLEAEVAPGFGRTMSPYPAETFRFPASPGPQQALMPPNLWSLRAKPGTARLPGEDMRGQWRPLSVEDIGAYSYPVSAAGRASPCSFSERYYGGAGGSPGKKADGRASPLYASYKADSFSEGDDLSQGHLAEPCFLRAGGDLSLSPGRSADPLPGYAPSEGGDGDRLGVQLCGTASSPEPEQGSRDSLEPSSMEASPEMHPAARLSPQQAFPRTGGSGLSRKDSLTKAQLYGTLLN

相同性解析による交差性が予測される生物種
Full Name brain-enriched guanylate kinase-associated homolog (rat)
Calculated molecular weight 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC002607
Gene Symbol BEGAIN
Gene ID (NCBI) 57596
RRIDAB_2064487
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for BEGAIN antibody 14956-1-APDownload protocol
WB protocol for BEGAIN antibody 14956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

The First Nationwide Survey and Genetic Analyses of Bardet-Biedl Syndrome in Japan.

Authors - Makito Hirano
View Article
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