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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BDH2 Monoclonal antibody (68279-1-Ig)

    WB analysis using 68279-1-Ig (same clone as 68279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BDH2 Monoclonal antibody (68279-1-Ig)

    WB analysis of HeLa using 68279-1-Ig (same clone as 68279-1-PBS)

    WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BDH2 Monoclonal antibody (68279-1-Ig)

    IF Staining of HepG2 using 68279-1-Ig (same clone as 68279-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BDH2 Monoclonal antibody, PBS Only

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68279-1-PBS
Clone No.1B6H5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

(R)-beta-hydroxybutyrate dehydrogenase, 1B6H5, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68279-1-PBS targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26108

Product name: Recombinant human BDH2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-245 aa of BC095414

Sequence: MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFANEVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL

相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Observed molecular weight 27 kDa
GenBank accession numberBC095414
Gene Symbol BDH2
Gene ID (NCBI) 56898
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BUT1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.