Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | NCCIT cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
60080-1-Ig targets BCRP/ABCG2 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BCRP/ABCG2 fusion protein Ag4869 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family G (WHITE), member 2 |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 70-80 kDa, 140-160 kDa |
| GenBank accession number | BC021281 |
| Gene Symbol | ABCG2 |
| Gene ID (NCBI) | 9429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q9UNQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Diosmetin Maintains Barrier Integrity by Reducing the Expression of ABCG2 in Colonic Epithelial Cells | ||
Fish Shellfish Immunol An ATP-binding cassette transporter G2 (CgABCG2) regulates the haemocyte proliferation by modulating the G1/S phase transition of cell cycle in oyster Crassostrea gigas | ||
J Nutr Biochem ABCG2 plays a central role in the dysregulation of 25-hydrovitamin D in Crohn's disease |