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Validation Data Gallery

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  • Western Blot (WB) analysis of SW480 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of SW480 using 68611-1-Ig (same clone as 68611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis using 68611-1-Ig (same clone as 68611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of RAW 264.7 using 68611-1-Ig (same clone as 68611-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of HEK-293 using 68611-1-Ig (same clone as 68611-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68611-1-PBS in a different storage buffer formulation.

BCOR Monoclonal antibody, PBS Only

BCOR Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68611-1-PBS
Clone No.1F12H6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

ANOP2, BCOR, FLJ20285, FLJ38041, KIAA1575, MAA2, MCOPS2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68611-1-PBS targets BCOR in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33785

Product name: Recombinant human BCOR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2-361 aa of BC009675

Sequence: RFRKRPEPSSDYDLSPAKQEPKPFDRLQQLLPASQSTQLPCSSSPQETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKICDVNHRDNAGYCALHEACARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLATYSGRTIMKMTHSELMEKFLTDYLNDLQGRNDDDASGTWDFYGSSVCEPDDESGYDVLANPPGPEDQDDDDDAYSDVFEFEFSETPLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIFRCNFPNVEIVTIAEAEFYRQVSASLLFSCSKDLEAFNPESKELLDLVEFTNEIQTLLGSSVEWLHPSDLASDNYW

相同性解析による交差性が予測される生物種
Full Name BCL6 co-repressor
Calculated molecular weight 1703 aa, 186 kDa
Observed molecular weight186-200 kDa
GenBank accession numberBC009675
Gene Symbol BCOR
Gene ID (NCBI) 54880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ6W2J9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem.