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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCO2 Polyclonal antibody (14324-1-AP)

    WB analysis using 14324-1-AP (same clone as 14324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using BCO2 Polyclonal antibody (14324-1-AP)

    WB analysis of L02 using 14324-1-AP (same clone as 14324-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using BCO2 Polyclonal antibody (14324-1-AP)

    WB analysis of mouse liver using 14324-1-AP (same clone as 14324-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using BCO2 Polyclonal antibody (14324-1-AP)

    IHC staining of human prostate hyperplasia using 14324-1-AP (same clone as 14324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using BCO2 Polyclonal antibody (14324-1-AP)

    IHC staining of human prostate hyperplasia using 14324-1-AP (same clone as 14324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

BCO2 Polyclonal antibody, PBS Only

BCO2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14324-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14324-1-PBS targets BCO2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5590

Product name: Recombinant human BCO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-506 aa of BC041656

Sequence: ERFMSRFELPGKAAGFSYKVIRVPPEKVDLGETIHGVQVICSIASTEKGKPSYYHSFGMTRNYIIFIEQPLKMNLWKIATSKIRGKAFSDGISWEPQCNTRFHVVEKRTGQLLPGRYYSKPFVTFHQINAFEDQGCVIIDLCCQDNGRTLEVYQLQNLRKAGEGLDQVHNSAAKSFPRRFVLPLNVSLNAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYDRFSGKKYHFFYGCGFRHLVGDSLIKVDVVNKTLKVWREDGFYPSEPVFVPAPGTNEEDGGVILSVVITPNQNESNFLLVLDAKNFEELGRAEVPVQMPYGFHGTFIPI

相同性解析による交差性が予測される生物種
Full Name beta-carotene oxygenase 2
Calculated molecular weight 66 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC041656
Gene Symbol BCO2
Gene ID (NCBI) 83875
RRIDAB_2227901
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYV7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

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