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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL9L Polyclonal antibody (32103-1-AP)

    WB analysis using 32103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32103-1-AP (BCL9L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BCL9L Polyclonal antibody (32103-1-AP)

    IF Staining of MCF-7 using 32103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BCL9L antibody (32103-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

BCL9L Polyclonal antibody

BCL9L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32103-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Nuclear Co-Factor Of Beta-Catenin Signalling, DLNB11, BCL9-like protein, B-cell lymphoma 9-like protein, B-cell CLL/lymphoma 9-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32103-1-AP targets BCL9L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL9L fusion protein Ag36794 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 9-like
Calculated molecular weight157 kDa
Observed molecular weight220 kDa
GenBank accession numberNM_182557
Gene Symbol BCL9L
Gene ID (NCBI) 283149
RRIDAB_3670192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86UU0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B-cell CLL/lymphoma 9-like protein (BCL9L, also known as B9L, BCL9-2, and DLNB11) is a transcriptional regulator that acts as an activator, which locates in nucleolus and nucleoplasm (PMID: 33767438). It is predicted to be implicated in the WNT-β-catenin signaling pathway to regulate the progress of hepatocellular carcinoma, breast cancer, and colon cancer (PMID: 31440992; 34545187; 30698996).

Protocols

Product Specific Protocols
WB protocol for BCL9L antibody 32103-1-APDownload protocol
IF protocol for BCL9L antibody 32103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols