Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 23959-1-AP (same clone as 23959-1-PBS)
WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 23959-1-AP (same clone as 23959-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)
IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 23959-1-AP (same clone as 23959-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

BCL3 Polyclonal antibody, PBS Only

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 23959-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23959-1-PBS targets BCL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21040 Product name: Recombinant human BCL3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 97-446 aa of BC064993 Sequence: NLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS 相同性解析による交差性が予測される生物種
Full Name	B-cell CLL/lymphoma 3
Calculated molecular weight	446 aa, 47 kDa
Observed molecular weight	48-60 kDa
GenBank accession number	BC064993
Gene Symbol	BCL3
Gene ID (NCBI)	602
RRID	AB_2879375
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20749
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248). The predicted molecular weight of the BCL3 protein is 48 kDa. However, due to post-translational modifications (e.g., phosphorylation), its apparent molecular weight often ranges between 55 and 65 kDa (PMID: 9407099, PMID: 12847243).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 23959-1-AP (same clone as 23959-1-PBS)

WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

WB analysis of A549 using 23959-1-AP (same clone as 23959-1-PBS)

IP experiment of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

IF Staining of HepG2 using 23959-1-AP (same clone as 23959-1-PBS)

BCL3 Polyclonal antibody, PBS Only

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™