Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IF | See 1 publications below |
Product Information
16026-1-AP targets BCL2L2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8863 Product name: Recombinant human BCL2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC021198 Sequence: MATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGALEEARRLREGNWASVR 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2-like 2 |
| Calculated molecular weight | 193 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC021198 |
| Gene Symbol | BCL2L2 |
| Gene ID (NCBI) | 599 |
| RRID | AB_2827646 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92843 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BCL2L2 antibody 16026-1-AP | Download protocol |
| WB protocol for BCL2L2 antibody 16026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting anti-apoptotic pathways eliminates senescent melanocytes and leads to nevi regression | ||
Neural Regen Res Mechanism of piR-1245/PIWI-like protein-2 regulating Janus kinase-2/signal transducer and activator of transcription-3/vascular endothelial growth factor signaling pathway in retinal neovascularization | ||
Oxid Med Cell Longev Peptides Isolated from Yak Milk Residue Exert Antioxidant Effects through Nrf2 Signal Pathway. | ||
Eur J Pharmacol GSK-3β-mediated activation of NLRP3 inflammasome leads to pyroptosis and apoptosis of rat cardiomyocytes and fibroblasts. | ||
Int J Mol Sci Targeting Bcl-xL with Navitoclax Effectively Eliminates Senescent Tumor Cells That Appear Following CEP-1347-Induced Differentiation of Glioma Stem Cells | ||
Int J Mol Sci Role of Orai3-Mediated Store-Operated Calcium Entry in Radiation-Induced Brain Microvascular Endothelial Cell Injury |