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Validation Data Gallery

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  • Western Blot (WB) analysis of SW480 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    WB analysis of SW480 using 23975-1-AP (same clone as 23975-1-PBS)

    WB result of BCL2L15 antibody (23975-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL2L15 transfected SW480 cells. This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon using 23975-1-AP (same clone as 23975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP (same clone as 23975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon cancer using 23975-1-AP (same clone as 23975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP (same clone as 23975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP (same clone as 23975-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP (same clone as 23975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

BCL2L15 Polyclonal antibody, PBS Only

BCL2L15 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 23975-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Bcl-2 family kin, Bcl2-L-15, Bcl-2-like protein 15, Bfk, C1orf178

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23975-1-PBS targets BCL2L15 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21155

Product name: Recombinant human BCL2L15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-163 aa of BC127719

Sequence: MKSSQTFEEQTECIVNTLLMDFLSPTLQVASRNLCCVDEVDSGEPCSFDVAIIAGRLRMLGDQFNGELEASAKNVIAETIKGQTGAILQNTVESLSKTWCAQDSSLAYERAFLAVSVKLLEYMAHIAPEVVGQVAIPMTGMINGNQAIREFIQGQGGWENLES

相同性解析による交差性が予測される生物種
Full Name BCL2-like 15
Calculated molecular weight 163 aa, 18 kDa
GenBank accession numberBC127719
Gene Symbol BCL2L15
Gene ID (NCBI) 440603
RRIDAB_3085718
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5TBC7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

B-cell lymphoma 2 (Bcl-2)-like protein 15 (BCL2L15), also known as BCL2 family kin (BFK), belongs to the BCL2 protein family, and appears to induce weak apoptosis and antagonize the pro-survival function of Bcl2 (PMID:32708974). In addition, studies reported BCL2L15 expression in the stomach, ovaries, bone marrow, spleen, and uterus (PMID:12700646).

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