• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL2L14 Polyclonal antibody

BCL2L14 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12846-1-AP

Synonyms

Apoptosis regulator Bcl G, BCL2L14

Validation Data Gallery

Applications:
  • WB analysis of A549 using 12846-1-AP

    WB analysis of A549 using 12846-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 12846-1-AP

    WB analysis of human lung using 12846-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12846-1-AP

    IHC staining of human prostate cancer using 12846-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12846-1-AP (BCL2L14 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of MCF-7 using 12846-1-AP

    IF Staining of MCF-7 using 12846-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12846-1-AP (BCL2L14 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, human lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12846-1-AP targets BCL2L14 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L14 fusion protein Ag3926 相同性解析による交差性が予測される生物種
Full Name BCL2-like 14 (apoptosis facilitator)
Calculated molecular weight 327 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC025778
Gene symbol BCL2L14
Gene ID (NCBI) 79370
RRIDAB_2290479
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCL2L14 antibody 12846-1-APDownload protocol
IHC protocol for BCL2L14 antibody 12846-1-APDownload protocol
IF protocol for BCL2L14 antibody 12846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols