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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL2 Recombinant antibody, PBS Only

BCL2 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O5

Cat no : 80313-1-PBS

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

Validation Data Gallery

Applications:
  • WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of human appendicitis using 80313-1-RR (same clone as 80313-1-PBS)

    IF Staining of human appendicitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

80313-1-PBS targets BCL2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).