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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL2 Recombinant antibody (80313-1-RR)

    WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL2 Recombinant antibody (80313-1-RR)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL2 Recombinant antibody (80313-1-RR)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using BCL2 Recombinant antibody (80313-1-RR)

    IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using BCL2 Recombinant antibody (80313-1-RR)

    IF Staining of human appendicitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80313-1-RR

    Affinity and Kinetic Characterization of 80313-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

BCL2 Recombinant monoclonal antibody, PBS Only

BCL2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 80313-1-PBS
Clone No.3O5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

BCL-2, 3O5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80313-1-PBS targets BCL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3508

Product name: Recombinant human BCL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC027258

Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK

相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2918886
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

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