Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells |
Positive IHC detected in | human tonsillitis tissue, human gliomas tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11613-1-AP targets BCL11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag2187 Product name: Recombinant human BCL11A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC021098 Sequence: MSRRKQGKPQHLSKREFSPEPLEAILTDDEPDHGPLGAPEGDHDLLTCGQCQMNFPLGDILIFIEHKRKQCNGSLCLEKAVDKPPSPSPIEMKKASNPVEVGIQVTPEDDDCLSTSSRGICPKQEHIADKLLHWRGLSSPRSAHGALIPTPGMSAEYAPQGICKDEPSSYTCTTCKQPFTSAWFLLQHAQNTHGLRIYLESEHGSPLTPRVGIPSGLGAECPSQPPLHGIHIADNNPFNLLRIPGSVSREASGLAEGRFPPTPPLFSPPPRHHLDPHRIERLGAEEMALATHHPSAFDRVLRLNPMAMEPPAMDFSRRLRELAGNTSSPPLSPGRPSPMQRLLQPFQPGSK 相同性解析による交差性が予測される生物種 |
Full Name | B-cell CLL/lymphoma 11A (zinc finger protein) |
Calculated molecular weight | 835 aa, 91 kDa |
GenBank accession number | BC021098 |
Gene Symbol | BCL11A |
Gene ID (NCBI) | 53335 |
RRID | AB_2259028 |
Conjugate | Unconjugated |
Form | |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H165 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
B-CELL CLL/LYMPHOMA 11A(BCL11A)is a highly conserved zinc finger gene. It acts as a myeloid and B-cell proto-oncogene, and may play key roles in leukemogenesis and hematopoiesis. It is an essential factor in lymphopoiesis, and required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. It is also involved in lymphoid malignancies through translocation or amplification events. It has recently been shown that BCL11A expression is inversely correlated with γ globin expression.(PMID:21156846). This is a rabbit polyclonal antibody raised against part chain of N-terminal BCL11A of human origin. There are three isoforms of BCL11A: isoform1(773aa), isoform2(486aa) and isoform3(239aa)
Protocols
Product Specific Protocols | |
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WB protocol for BCL11A antibody 11613-1-AP | Download protocol |
IHC protocol for BCL11A antibody 11613-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |