Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells |
| Positive IHC detected in | Breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65014-1-Ig targets BCL10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | B-cell CLL/lymphoma 10 |
| Calculated molecular weight | 233 aa, 26 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC053617 |
| Gene Symbol | BCL10 |
| Gene ID (NCBI) | 8915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95999 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. |
Background Information
The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.