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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCKDK Polyclonal antibody

BCKDK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15718-1-AP

Synonyms

BCKDK

Validation Data Gallery

Applications:
  • WB analysis of mouse pancreas using 15718-1-AP

    WB analysis of mouse pancreas using 15718-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 15718-1-AP

    IHC staining of human heart using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included
  • IHC staining of human heart using 15718-1-AP

    IHC staining of human heart using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCKDK Ready-To-Use IHC Kit. BCKDK primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15718-1-AP targets BCKDK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCKDK fusion protein Ag8395 相同性解析による交差性が予測される生物種
Full Name branched chain ketoacid dehydrogenase kinase
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC007363
Gene symbol BCKDK
Gene ID (NCBI) 10295
RRIDAB_2878174
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCKDK antibody 15718-1-APDownload protocol
IHC protocol for BCKDK antibody 15718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.

Authors - Yang Yang
View Article